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Home >> Antibodies >> ErbB2 Antibody

ErbB2 Antibody (F50600)

  Catalog No Formulation Size Price (USD)  
Image F50600-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50600-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of ErbB2 in T47D cell lysate, either noninduced (Lane 1) or induced with HRG (2).
Flow cytometric analysis of MCF-7 cells using HER2 / ErbB2 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human breast carcinoma tissue stained with ErbB2 antibody
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P04626
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
IHC (Paraffin) : 1:50-1:100
Limitations This ErbB2 antibody is available for research use only.
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Description

ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An Interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues

Application Notes

Titration of the ErbB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 21-52 from the human protein was used as the immunogen for this ErbB2 antibody.

Storage

Aliquot the ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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