ERAL1 Antibody / GTPase Era, mitochondrial Antibody (FY13643)

ERAL1 Antibody by WB in human cell lines. Western blot analysis using GTPase Era, mitochondrial Antibody detects a band near 39-40 kDa in three human cell lysates. Lane 1 contains HEK293T cells, lane 2 contains K562 cells, and lane 3 contains A549 cells. The bands migrate at similar apparent molecular weights across the three samples, consistent with detection of ERAL1. The blot includes a molecular weight marker and was developed by immunoblotting with an anti-ERAL1 primary antibody. This experiment documents detection of a target-sized protein in multiple human cellular backgrounds.

ERAL1 Antibody by IHC in human ovarian cancer tissue. Immunohistochemistry using GTPase Era, mitochondrial Antibody shows brown DAB staining in a paraffin-embedded section of human ovarian carcinoma. The image includes hematoxylin-counterstained nuclei and heterogeneous staining among the tumor cells. Heat-induced epitope retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with goat serum. The tissue was incubated with rabbit anti-ERAL1 antibody at 2 ug/mL overnight at 4oC, and signal was developed using an HRP-based detection system with DAB chromogen.

ERAL1 Antibody by IP from K562 cell lysate. Immunoprecipitation analysis using GTPase Era, mitochondrial Antibody shows an ERAL1-reactive band in the antibody immunoprecipitate. The figure includes an input lysate lane and a control-IgG immunoprecipitation lane for comparison. K562 cell lysate was used as the starting material, and the recovered material was analyzed by immunoblotting. The visible band in the antibody-IP lane supports enrichment of an ERAL1-reactive protein under the tested conditions.