ER Antibody Clone ER506 | Estrogen Receptor

	Catalog No	Formulation	Size	Price (USD)
	V2113-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V2113-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V2113SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

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Immunohistochemistry of ER Antibody in human endometrium. Formalin-fixed, paraffin-embedded human endometrial tissue shows strong nuclear HRP-DAB brown staining in glandular epithelial cells and stromal cells, consistent with Estrogen Receptor expression, while surrounding non-epithelial elements show reduced staining intensity. Staining was performed using clone ER506 following heat-induced epitope retrieval according to standard protocols. The nuclear staining pattern aligns with expected Estrogen Receptor alpha / ESR1 localization in hormonally responsive endometrial tissue.

Intracellular FACS testing of human MCF-7 cells: Black=cells alone; Green=isotype control; Red= PE conjugated ER antibody (V2113PE)

Western blot testing of MCF-7 cell lysate with ER antibody (clone ER506). Predicted molecular weight of ER alpha: ~67 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ER antibody (clone ER506). These results demonstrate the foremost specificity of the ER506 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free ER antibody (clone ER506) as confirmation of integrity and purity.

Immunofluorescent staining of methanol-fixed human MCF7 cells with ER antibody (clone ER506, green) and Reddot nuclear stain (red).

Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	ER506
Purity	Protein G affinity chromatography
Gene ID	2099
Localization	Intracellular, Nuclear
Applications	Flow Cytometry : 1-2ug/10^6 cells Western Blot : 2-4ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml (1) Immunofluorescence : 1-3ug/ml
Limitations	This ER antibody is available for research use only.
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Description

ER Antibody Clone ER506 recognizes Estrogen Receptor alpha, a ligand-activated nuclear transcription factor encoded by the ESR1 gene on chromosome 6q25.1. Estrogen Receptor alpha, commonly referred to as ER alpha or ESR1, is a member of the nuclear receptor superfamily that regulates gene expression in response to estrogen signaling. ER Antibody Clone ER506 has been widely utilized in peer-reviewed scientific literature, making this clone a well-established reagent for studies involving hormone receptor biology and estrogen-dependent signaling pathways.

Estrogen Receptor alpha contains a conserved DNA-binding domain with zinc finger motifs, a ligand-binding domain responsible for estrogen interaction, and activation function domains that coordinate transcriptional regulation. Upon ligand binding, ESR1 undergoes conformational changes that promote receptor dimerization and nuclear localization. The activated receptor binds estrogen response elements within promoter regions of target genes and recruits transcriptional coactivators or corepressors, thereby regulating genes that control cellular proliferation, differentiation, and survival.

Expression of ER alpha is most prominent in breast epithelium, endometrium, ovary, and additional hormone-responsive tissues. In oncology research, ESR1 expression status serves as a critical biomarker in breast carcinoma and other estrogen-dependent malignancies. Alterations in ESR1 signaling, including amplification, splice variants, or activating mutations, can influence tumor growth behavior and endocrine responsiveness. Beyond oncology, ER alpha also contributes to bone density regulation, cardiovascular physiology, and neuroendocrine function.

ER Antibody clone ER506 has been referenced across numerous independent studies, supporting its established presence in the research community. The clone-specific differentiation provides investigators with a reagent that has demonstrated broad utility in published experimental contexts. By targeting Estrogen Receptor alpha protein, this antibody supports research focused on estrogen-driven transcriptional programs and hormone-dependent disease mechanisms.