EPS8L2 Antibody / EPS8 signaling adaptor L2 Antibody (FY13637)

EPS8L2 Antibody by IF in A431 cells. Immunofluorescence analysis using EPS8 signaling adaptor L2 Antibody shows green fluorescence predominantly in the cytoplasm and cell periphery of A431 cells, with DAPI-stained nuclei in blue. Cells underwent enzyme antigen retrieval and were incubated with 5 ug/mL primary antibody overnight at 4oC, followed by a fluorescent secondary antibody. The distribution is compatible with a role in actin-associated cellular structures, although no cytoskeletal colocalization marker is included.

EPS8L2 Antibody by IHC in human placenta. Immunohistochemistry of paraffin-embedded human placenta using EPS8 signaling adaptor L2 Antibody reveals brown DAB staining in placental villous structures, with hematoxylin-counterstained nuclei. Sections underwent EDTA antigen retrieval at pH 8.0 and were incubated with 2 ug/mL primary antibody overnight at 4oC before peroxidase-based visualization.

EPS8L2 Antibody by WB in human cell lines. Western blot analysis using EPS8 signaling adaptor L2 Antibody detects a prominent band consistent with the expected molecular weight of approximately 81 kDa in human HepG2 (lane 1), HeLa (lane 2), and A549 (lane 3) whole-cell lysates. Reduced samples containing 30 ug protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, membranes were incubated with 0.5 ug/mL primary antibody overnight at 4oC. Bound antibody was visualized using an HRP-conjugated secondary antibody and chemiluminescent substrate.

EPS8L2 Antibody by IP in HepG2 cells. Immunoprecipitation of EPS8L2 from human HepG2 whole-cell lysate was assessed by Western blot using EPS8 signaling adaptor L2 Antibody. Lane 1 contains input lysate (30 ug), lane 2 the rabbit IgG control immunoprecipitate, and lane 3 the specific immunoprecipitate prepared using 2 ug antibody and 500 ug lysate. A band consistent with the expected approximately 81 kDa protein is present in input and the specific immunoprecipitate. The approximately 50 kDa band in the control lane is consistent with immunoglobulin heavy chain.