EPS8L1 Antibody / EPS8 signaling adaptor L1 Antibody (FY13654)

EPS8L1 Antibody by WB in 3 lysate samples. Western blot analysis using EPS8L1 Antibody was performed with 30 ug of protein per lane under reducing conditions on a 10% SDS-PAGE gel. Lane assignments: 1, human HCT116 whole-cell lysate; 2, human MCF-7 whole-cell lysate; 3, human Caco-2 whole-cell lysate. Following electrophoresis, proteins were transferred to nitrocellulose and blocked with 5% non-fat milk. The membrane was incubated with rabbit primary antibody at 0.5 ug/ml overnight at 4oC and HRP-conjugated goat anti-rabbit IgG secondary antibody (1:5000). ECL detection showed an immunoreactive band near 85 kDa, consistent with the approximately 80 kDa expected size of EPS8L1.