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Home >> Antibodies >> EPO Antibody / Erythropoietin

EPO Antibody / Erythropoietin [clone EPO/1368] (V3373)

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  Catalog No Formulation Size Price (USD)  
Image V3373-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3373-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3373SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of with EPO antibody (clone EPO/1368). Predicted molecular weight: 18-34 kDa depending on glycosylation level.
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Western blot testing of with EPO antibody (clone EPO/1368). Predicted molecular weight: 18-34 kDa depending on glycosylation level.
SDS-PAGE Analysis of Purified, BSA-Free EPO Antibody (clone EPO/1368). Confirmation of Integrity and Purity of the Antibody.
IHC staining of FFPE human renal cell carcinoma with EPO antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using EPO antibody (clone EPO/1368). These results demonstrate the foremost specificity of the EPO/1368 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name EPO/1368
Purity Protein G affinity chromatography
UniProt P01588
Localization Cell surface, cytoplasmic
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This EPO antibody is available for research use only.
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Description

Erythropoietin / EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.

Application Notes

Titering of the EPO antibody may be required for optimal performance.

1. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Immunogen

A human recombinant partial protein was used as the immunogen for the EPO antibody.

Storage

Store the EPO antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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