Epidermal Growth Factor-Like Protein 7 Antibody / EGFL7 [clone EGFL7/9499] (V5966)

Immunohistochemistry analysis of Epidermal Growth Factor-Like Protein 7 / EGFL7 antibody in human endometrium. FFPE human endometrium tissue was stained with Epidermal Growth Factor-Like Protein 7 / EGFL7 antibody (clone EGFL7/9499). HRP-DAB brown chromogenic signal is observed predominantly along the luminal and glandular epithelial surfaces, with membranous and cytoplasmic staining of epithelial cells. The staining pattern is consistent with extracellular and endothelial-associated localization of EGFL7 within glandular structures. Surrounding stromal cells show comparatively weaker signal. Nuclei are counterstained blue. The inset image shows PBS used instead of primary antibody, demonstrating absence of specific HRP-DAB brown staining and confirming staining specificity. Heat-induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to antibody incubation.

Western blot analysis of Epidermal Growth Factor-Like Protein 7 / EGFL7 antibody in human tissues and cell lines. Western blot was performed using Epidermal Growth Factor-Like Protein 7 / EGFL7 antibody (clone EGFL7/9499) on human kidney, human lung, human spleen, HL60, K-562, and MCF7 lysates. A distinct immunoreactive band is detected at approximately 27 kDa across all tested samples, consistent with the predicted molecular weight of EGFL7. Band intensity varies slightly among tissues and cell lines, reflecting differential endogenous expression levels. The detected signal corresponds to the predicted molecular weight under reducing conditions. EGFL7 is a secreted extracellular matrix-associated protein expressed primarily by endothelial cells, and the observed band pattern supports specific recognition of endogenous EGFL7 in human tissues and cultured cells.

Western blot analysis of Epidermal Growth Factor-Like Protein 7 / EGFL7 antibody in 293T lysate. Western blot was performed using Epidermal Growth Factor-Like Protein 7 / EGFL7 antibody (clone EGFL7/9499) on human 293T whole cell lysate. A distinct immunoreactive band is observed at approximately 20 kDa. The predicted molecular weight of EGFL7 is approximately 30 kDa; however, EGFL7 is a secreted extracellular matrix-associated protein that can undergo proteolytic processing, which may account for the lower apparent molecular weight observed under reducing conditions.

SDS-PAGE Analysis of Purified Epidermal Growth Factor-Like Protein 7/EGFL7 antibody (EGFL7/9499). Confirmation of Purity and Integrity of Antibody.