EPHB4 Antibody / Receptor Tyrosine Kinase Antibody [clone EPHB4/6392] (V5211)

EPHB4 Antibody Liver Metastasis Colon IHC. Immunohistochemistry analysis of FFPE human liver metastasis in colon tissue using EPHB4 Antibody (clone EPHB4/6392) demonstrates HRP-DAB brown membranous and cytoplasmic staining in tumor cells, consistent with Eph receptor B4 / EPHB4 expression as a receptor tyrosine kinase involved in tumor signaling and vascular interactions, while surrounding stromal components show lower signal; nuclei are counterstained blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

EPHB4 Antibody Human Lung IHC. Immunohistochemistry analysis of FFPE human lung tissue using EPHB4 Antibody (clone EPHB4/6392) demonstrates HRP-DAB brown membranous and cytoplasmic staining in epithelial and vascular-associated cells, consistent with Eph receptor B4 / EPHB4 expression as a receptor tyrosine kinase involved in cell signaling and vascular biology, while surrounding stromal elements show lower signal; nuclei are counterstained blue. Inset: PBS used in place of primary antibody shows minimal background staining, confirming specificity. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

EPHB4 Antibody Microarray Specificity Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using EPHB4 Antibody (clone EPHB4/6392) demonstrates highly specific detection of Eph receptor B4 / EPHB4, a receptor tyrosine kinase involved in vascular and tumor signaling pathways. The antibody shows a dominant signal for EPHB4 with clear separation from other proteins on the array, supporting strong target specificity of clone EPHB4/6392. Z- and S-score: The Z-score represents the strength of signal generated when the antibody binds to a protein on the array, expressed as standard deviations above the mean signal, while the S-score reflects the difference between sequential Z-scores and indicates relative specificity compared to potential off-target interactions.