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Home >> Antibodies >> Eph Receptor B2 Antibody [EPHB2]

Eph Receptor B2 Antibody [EPHB2] [clone 48CT12.6.4] (F40413)

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  Catalog No Formulation Size Price (USD)  
Image F40413-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F40413-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Western blot analysis partial EPHB2 protein using EphB2 antibody at 1:1000.
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Western blot analysis partial EPHB2 protein using EphB2 antibody at 1:1000.
Flow cytometric analysis of HepG2 cells using EPHB2 antibody (red) compared to a negative control (blue). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
IHC analysis of FFPE human lung carcinoma tissue stained with EPHB2 antibody
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, k
Clone Name 48CT12.6.4
Purity Purified
UniProt P29323
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
IHC (Paraffin) : 1:200
Limitations This EPHB2 antibody is available for research use only.
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Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Application Notes

Titration of the EPHB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Purified His-tagged protein (from amino acids 127~425) was used to produce this monoclonal EPHB2 antibody.

Storage

Aliquot the EPHB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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