EPHA2 Antibody / Receptor Tyrosine Kinase Antibody (RQ4038)

EPHA2 Antibody Multi-Cell Line WB. Western blot analysis of EPHA2 expression was performed using an Eph Receptor A2 antibody. Lane 1: human HeLa cell lysate. Lane 2: human U-87 MG cell lysate. Lane 3: human SHG-44 cell lysate. Lane 4: human COLO320 cell lysate. Lane 5: human SKOV3 cell lysate. Lane 6: human A549 cell lysate. Lane 7: mouse HEPA1-6 cell lysate. EPHA2, also known as Epithelial Cell Kinase and a Receptor Tyrosine Kinase, regulates cell adhesion, migration, and signal transduction through interactions with ephrin ligands. A prominent band is detected between approximately 108 and 130 kDa, consistent with the expected molecular weight of mature glycosylated EPHA2. Additional lower molecular weight species observed in A549 and HEPA1-6 cells may represent proteolytic fragments or differentially processed receptor forms. These results demonstrate broad EPHA2 expression across epithelial, neural, ovarian, pulmonary, and hepatic cell types and support the utility of Eph Receptor A2 Antibody for studies of cancer biology and receptor signaling.

EPHA2 Antibody A549 Cells FACS. Flow cytometric analysis of human A549 cells was performed using an Eph Receptor A2 antibody at 1 ug per 10^6 cells following blocking with goat sera. EPHA2, also known as Epithelial Cell Kinase and a Receptor Tyrosine Kinase, regulates cell adhesion, migration, and growth through interactions with membrane-bound ephrin ligands. Red histogram: cells alone. Green histogram: isotype control. Blue histogram: Eph Receptor A2 antibody. The pronounced rightward shift of the EPHA2 antibody signal relative to both controls demonstrates specific detection of endogenous EPHA2 expression in A549 lung adenocarcinoma cells. Elevated EPHA2 expression has been implicated in tumor progression and therapeutic resistance in multiple cancers. These results support the utility of Eph Receptor A2 Antibody for studies of receptor tyrosine kinase signaling, cell migration, and cancer biology.

EPHA2 Antibody U-2 OS Cells FACS. Flow cytometric analysis of human U-2 OS cells was performed using an Eph Receptor A2 antibody at 1 ug per 10^6 cells following blocking with goat sera. EPHA2, also known as Epithelial Cell Kinase and a Receptor Tyrosine Kinase, is a cell surface receptor that regulates cell adhesion, migration, proliferation, and tissue organization through interactions with ephrin ligands. Red histogram: cells alone. Green histogram: isotype control. Blue histogram: Eph Receptor A2 antibody. The marked rightward shift of the antibody signal relative to both controls demonstrates specific detection of endogenous EPHA2 expression in U-2 OS osteosarcoma cells. Elevated EPHA2 signaling has been implicated in tumor progression, invasion, and metastatic potential. These results support the utility of Eph Receptor A2 Antibody for studies of receptor tyrosine kinase signaling, cell migration, and cancer biology.

EPHA2 Antibody PC-3 Cells IF. Immunofluorescent analysis of EPHA2 expression was performed in human PC-3 cells using an anti-EPHA2 antibody. EPHA2, also known as Eph Receptor A2 and a Receptor Tyrosine Kinase, regulates cell adhesion, migration, and epithelial organization through interactions with ephrin ligands. Strong cytoplasmic and membrane-associated fluorescence is observed throughout the cells, consistent with the localization and trafficking of this cell surface receptor. Cells were incubated with rabbit anti-EPHA2 antibody at 5 ug/ml and detected with Fluoro488-conjugated goat anti-rabbit IgG (green). Nuclei were counterstained with DAPI (blue). These results support the utility of EPHA2 Antibody for studies of receptor tyrosine kinase signaling, cell migration, and cancer biology.