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Home >> Antibodies >> Eph Receptor A2 Antibody

Eph Receptor A2 Antibody (RQ4038)

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  Catalog No Formulation Size Price (USD)  
Image RQ4038 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) U-87 MG, 3) SHG-44, 4) COLO320, 5) SKOV3, 6) A549 and 7) mouse HEPA1-6 cell lysate with Eph Receptor A2 antibody at 0.5ug/ml. Expected molecular weight: 108~130 kDa.
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Western blot testing of human 1) HeLa, 2) U-87 MG, 3) SHG-44, 4) COLO320, 5) SKOV3, 6) A549 and 7) mouse HEPA1-6 cell lysate with Eph Receptor A2 antibody at 0.5ug/ml. Expected molecular weight: 108~130 kDa.
Flow cytometry testing of human A549 cells with Eph Receptor A2 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eph Receptor A2 antibody.
Flow cytometry testing of human U-2 OS cells with Eph Receptor A2 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eph Receptor A2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P29317
Applications Western Blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/10^6 cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Eph Receptor A2 antibody is available for research use only.
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Description

EPHA2 (Ephrin type-A receptor 2) also known as ECK, is a protein that in humans is encoded by the EPHA2 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. By somatic cell hybrid analysis and fluorescence in situ hybridization, the EPHA2 gene is mapped to chromosome 1p36.1. EPHA2 was readily detectable in human lens fiber cells using immunoblot and immunohistochemistry. EGFR and EPHA2 mediated HCV entry by regulating CD81 -claudin-1 (CLDN1) coreceptor associations and viral glycoprotein-dependent membrane fusion.

Application Notes

Optimal dilution of the Eph receptor A2 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein corresponding to amino acids M851-N970 was used as the immunogen for the Eph Receptor A2 antibody.

Storage

After reconstitution, the Eph Receptor A2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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