EPDR1 Antibody / Mammalian ependymin-related protein 1 Antibody (FY13651)

EPDR1 Antibody by IHC in mouse brain tissue. Immunohistochemistry of paraffin-embedded mouse brain tissue using EPDR1 Antibody, with antigen retrieval in EDTA buffer (pH 8.0) and blocking in 10% goat serum. The section was incubated with rabbit primary antibody at 2 ug/ml overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB development. Brown DAB labeling is visible in scattered cells against blue counterstained nuclei. This experiment illustrates detection using the EPDR1 antibody under the indicated staining conditions.

EPDR1 Antibody by IHC in rat brain tissue. Immunohistochemistry of paraffin-embedded rat brain tissue using EPDR1 Antibody, with antigen retrieval in EDTA buffer (pH 8.0) and blocking in 10% goat serum. The section was incubated with rabbit primary antibody at 2 ug/ml overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB development. Brown reaction product is distributed through the examined brain section, with blue nuclear counterstaining. This experiment illustrates detection using the EPDR1 antibody under the indicated staining conditions.

EPDR1 Antibody by WB in 5 lysate samples. Western blot analysis using EPDR1 Antibody was performed with 30 ug of protein per lane under reducing conditions on a 12% SDS-PAGE gel. Lane assignments: 1, human A549 whole-cell lysate; 2, human HeLa whole-cell lysate; 3, human Jurkat whole-cell lysate; 4, rat brain lysate; 5, mouse brain lysate. Following electrophoresis, proteins were transferred to nitrocellulose and blocked with 5% non-fat milk. The membrane was incubated with rabbit primary antibody at 0.5 ug/ml overnight at 4oC and HRP-conjugated goat anti-rabbit IgG secondary antibody (1:5000). ECL detection showed an immunoreactive band near 25-27 kDa, consistent with the approximately 25 kDa expected size of EPDR1.

EPDR1 Antibody by IHC in human prostate cancer tissue. Immunohistochemistry of paraffin-embedded human prostate cancer tissue using EPDR1 Antibody, with antigen retrieval in EDTA buffer (pH 8.0) and blocking in 10% goat serum. The section was incubated with rabbit primary antibody at 2 ug/ml overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB development. Brown immunoreactivity is apparent in gland-forming tumor cells, with blue nuclear counterstaining. This experiment illustrates detection using the EPDR1 antibody under the indicated staining conditions.