Epcam Antibody / Mouse and Rat Reactive Antibody (RQ6173)

Epcam Antibody Mouse Intestine IF. Immunofluorescent staining of FFPE mouse intestine tissue using Epcam Antibody demonstrates strong red fluorescence localized predominantly to the epithelial lining of intestinal villi and crypt structures. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein involved in epithelial cell adhesion, maintenance of tissue architecture, and regulation of epithelial differentiation. Prominent membrane-associated fluorescence outlines the intestinal epithelial compartment and highlights the organized cellular structure of the mucosal lining. Nuclei are counterstained with DAPI (blue). The observed staining supports the use of Epcam as a marker for epithelial cell identification, intestinal epithelial biology, and studies of tissue organization and epithelial homeostasis in mouse tissues. HIER: steam tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining.

Epcam Antibody Mouse Intestine IHC. Immunohistochemistry staining of FFPE mouse intestine tissue using Epcam Antibody demonstrates strong membranous HRP-DAB brown staining throughout the epithelial lining of intestinal villi and crypt structures. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein involved in epithelial cell adhesion, maintenance of tissue architecture, and regulation of epithelial differentiation. Prominent membrane-associated staining outlines individual enterocytes and highlights the highly organized epithelial compartment of the intestinal mucosa. The observed immunoreactivity is consistent with the established expression of EPCAM in gastrointestinal epithelial tissues and supports the use of Epcam as a marker for epithelial cell identification, intestinal biology studies, and investigations of epithelial tissue homeostasis in mouse models. HIER: boil tissue sections in pH 8 EDTA buffer for 20 minutes and allow to cool before testing.

Epcam Antibody Rat Intestine IHC. Immunohistochemistry staining of FFPE rat intestine tissue using Epcam Antibody demonstrates distinct membranous HRP-DAB brown staining throughout the intestinal epithelial lining, including absorptive enterocytes and crypt-associated epithelial cells. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a cell surface glycoprotein that mediates epithelial cell adhesion and contributes to maintenance of epithelial architecture and tissue integrity. Prominent membrane localization highlights epithelial cell boundaries along the mucosal surface and is characteristic of EPCAM expression in gastrointestinal epithelial tissues. The observed immunoreactivity supports the use of Epcam as a marker for epithelial cell identification, intestinal epithelial differentiation, and studies of epithelial biology in rat models. HIER: boil tissue sections in pH 8 EDTA buffer for 20 minutes and allow to cool before testing.