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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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EpCAM antibody targets Epithelial cell adhesion molecule, encoded by the EPCAM gene. Epithelial cell adhesion molecule is a type I transmembrane glycoprotein predominantly localized to the cell surface of epithelial cells. It is a member of the EpCAM family of adhesion molecules and plays an important role in mediating cell-cell interactions, epithelial integrity, and tissue organization.
Epithelial cell adhesion molecule is best known for its function in calcium independent homophilic cell adhesion. Through its extracellular domain, EpCAM contributes to intercellular cohesion within epithelial layers, while its intracellular domain participates in signaling pathways that influence cell proliferation and differentiation. An EpCAM antibody supports research focused on epithelial biology, cell adhesion mechanisms, and membrane associated signaling processes.
EpCAM expression is largely restricted to epithelial tissues, where it is highly expressed in simple and stratified epithelia. It is frequently used as a marker of epithelial identity and is notably absent from mesenchymal and hematopoietic cells under normal physiological conditions. Subcellular localization of Epithelial cell adhesion molecule is primarily at the plasma membrane, although regulated proteolytic cleavage can release intracellular fragments involved in downstream signaling events.
From a disease relevance perspective, Epithelial cell adhesion molecule has been extensively studied in cancer biology. Aberrant EpCAM expression is observed in a wide range of epithelial derived tumors, where it has been associated with altered adhesion, enhanced proliferation, and changes in differentiation status. These properties have made EpCAM a widely used biomarker in studies of tumor progression, epithelial plasticity, and circulating tumor cell detection.
At the molecular level, Epithelial cell adhesion molecule contains an extracellular domain responsible for adhesion, a single transmembrane region, and a short cytoplasmic tail involved in intracellular signaling interactions. Its functional activity can be influenced by proteolytic processing and cellular context, which may affect its apparent behavior in experimental systems. EpCAM antibody, clone EGP40/1373, enables investigation of epithelial cell adhesion and membrane associated signaling pathways, with NSJ Bioreagents providing reagents intended for research use.
Optimal dilution of the EpCAM antibody should be determined by the researcher.
A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the EpCAM antibody.
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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