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Home >> Antibodies >> EpCAM Antibody

EpCAM Antibody [clone EGP40/1373] (V7930)

  Catalog No Formulation Size Price (USD)  
Image V7930-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7930-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7930SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated
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Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone EGP40/1373, green) and Reddot nuclear stain (red).
IHC staining of FFPE human fallopian tube with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human lung tissue lysate with EpCAM antibody. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using EpCAM antibody (clone EGP40/1373). These results demonstrate the foremost specificity of the EGP40/1373 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone EGP40/1373) as confirmation of integrity and purity.
IHC staining of FFPE human rectal mass with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE dog colon with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE dog bladder with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE cat small intestine with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Dog, Cat
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name EGP40/1373
Purity Protein G affinity chromatography
UniProt P16422
Localization Cell surface, cytoplasmic
Applications Western Blot : 1-2ug/ml
Immunofluorescence : 1-3ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This EpCAM antibody is available for research use only.
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Description

EpCAM antibody targets Epithelial cell adhesion molecule, encoded by the EPCAM gene. Epithelial cell adhesion molecule is a type I transmembrane glycoprotein predominantly localized to the cell surface of epithelial cells. It is a member of the EpCAM family of adhesion molecules and plays an important role in mediating cell-cell interactions, epithelial integrity, and tissue organization.

Epithelial cell adhesion molecule is best known for its function in calcium independent homophilic cell adhesion. Through its extracellular domain, EpCAM contributes to intercellular cohesion within epithelial layers, while its intracellular domain participates in signaling pathways that influence cell proliferation and differentiation. An EpCAM antibody supports research focused on epithelial biology, cell adhesion mechanisms, and membrane associated signaling processes.

EpCAM expression is largely restricted to epithelial tissues, where it is highly expressed in simple and stratified epithelia. It is frequently used as a marker of epithelial identity and is notably absent from mesenchymal and hematopoietic cells under normal physiological conditions. Subcellular localization of Epithelial cell adhesion molecule is primarily at the plasma membrane, although regulated proteolytic cleavage can release intracellular fragments involved in downstream signaling events.

From a disease relevance perspective, Epithelial cell adhesion molecule has been extensively studied in cancer biology. Aberrant EpCAM expression is observed in a wide range of epithelial derived tumors, where it has been associated with altered adhesion, enhanced proliferation, and changes in differentiation status. These properties have made EpCAM a widely used biomarker in studies of tumor progression, epithelial plasticity, and circulating tumor cell detection.

At the molecular level, Epithelial cell adhesion molecule contains an extracellular domain responsible for adhesion, a single transmembrane region, and a short cytoplasmic tail involved in intracellular signaling interactions. Its functional activity can be influenced by proteolytic processing and cellular context, which may affect its apparent behavior in experimental systems. EpCAM antibody, clone EGP40/1373, enables investigation of epithelial cell adhesion and membrane associated signaling pathways, with NSJ Bioreagents providing reagents intended for research use.

Application Notes

Optimal dilution of the EpCAM antibody should be determined by the researcher.

Immunogen

A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the EpCAM antibody.

Storage

Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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