EpCAM Antibody [clone EGP40/1373] (V7930)

EpCAM Antibody MCF-7 IF. Immunofluorescent staining of human MCF-7 breast carcinoma cells using EpCAM Antibody (clone EGP40/1373) demonstrates strong green fluorescence localized predominantly to the plasma membrane and cell-cell junctions. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein involved in epithelial cell adhesion, maintenance of tissue architecture, and regulation of cellular signaling pathways. Prominent membrane-associated fluorescence outlines individual cells and highlights epithelial cell boundaries, consistent with the established localization of EpCAM on the surface of epithelial-derived tumor cells. Nuclei are counterstained with Reddot (red). The observed staining supports the use of EpCAM as a marker for epithelial lineage identification, epithelial tumor cell characterization, and cell surface protein localization studies.

EpCAM Antibody Fallopian Tube IHC. Immunohistochemistry staining of FFPE human fallopian tube tissue using EpCAM Antibody (clone EGP40/1373) demonstrates strong membranous and apical HRP-DAB brown staining throughout the columnar epithelial lining of the mucosal folds. The staining pattern is consistent with expression of Epithelial Cell Adhesion Molecule (EPCAM/CD326), a transmembrane glycoprotein involved in epithelial cell adhesion, tissue organization, and maintenance of epithelial integrity. Prominent membrane-associated staining sharply delineates the highly folded epithelial architecture of the fallopian tube and is consistent with the established expression of EpCAM in normal glandular and mucosal epithelial tissues. The observed immunoreactivity supports the use of EpCAM as a marker for epithelial cell identification, epithelial differentiation studies, and characterization of epithelial-derived tissues. HIER: boil tissue sections in pH 9 Tris-EDTA buffer (10 mM Tris, 1 mM EDTA) for 10-20 minutes and allow to cool before testing.

Western blot testing of human lung tissue lysate with EpCAM antibody. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using EpCAM antibody (clone EGP40/1373). These results demonstrate the foremost specificity of the EGP40/1373 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone EGP40/1373) as confirmation of integrity and purity.

IHC staining of FFPE human rectal mass with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE dog colon with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE dog bladder with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE cat small intestine with EpCAM antibody (clone EGP40/1373). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.