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Home >> Antibodies >> EpCAM Antibody

EpCAM Antibody [clone CIB-5] (RQ5484)

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Image RQ5484 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 439
Microvalidated Rab Mono Image
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IHC staining of FFPE mouse small intestine tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse small intestine tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse small intestine tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat small intestine tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat small intestine tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue with EpCAM antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Caco-2, 2) human MCF7, 3) rat small intestine and 4) mouse small intestine tissue lysate with EpCAM antibody. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
Immunofluorescent staining of FFPE human HeLa cells with EpCAM antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-2 weeks
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CIB-5
Purity Affinity purified
UniProt P16422
Applications Western Blot : 1:1000-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence : 1:50
Limitations This EpCAM antibody is available for research use only.
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Description

The EPCAM gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [RefSeq]

Application Notes

Optimal dilution of the EpCAM antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human EpCAM was used as the immunogen for the EpCAM antibody.

Storage

Store the EpCAM antibody at -20oC.

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