EPC2 Antibody / Enhancer of polycomb homolog 2 Antibody (FY13646)

EPC2 Antibody by FCM in RT4 cells. Flow cytometry of fixed, permeabilized RT4 cells stained with EPC2 Antibody and a Fluoro488-conjugated secondary antibody. The blue EPC2 histogram is shifted to higher fluorescence than the green rabbit IgG isotype control, while the red unstained control remains at lower fluorescence. Cells were blocked with 10% goat serum and incubated with 1 ug of primary antibody per million cells. The histogram supports intracellular EPC2 detection under the tested conditions.

EPC2 Antibody by IHC in human brain tissue. Immunohistochemistry of paraffin-embedded human brain tissue using Enhancer of polycomb homolog 2 Antibody. Heat-induced epitope retrieval used EDTA buffer at pH 8.0, followed by 10% goat serum blocking. The section was incubated with 2 ug/ml primary antibody overnight at 4oC. Peroxidase-conjugated goat anti-rabbit IgG and DAB chromogen produced brown immunoreactivity, with blue nuclear counterstaining visible throughout the section.

EPC2 Antibody by IF in HeLa cells. Immunofluorescence staining of HeLa cells with Enhancer of polycomb homolog 2 Antibody, shown in green, together with alpha tubulin staining in red. The green signal is concentrated in nuclei, while the red tubulin signal outlines the cytoplasmic microtubule network. Cells were incubated with 5 ug/ml primary antibody overnight at 4oC, followed by fluorescent goat anti-rabbit and goat anti-mouse secondary antibodies.

EPC2 Antibody by WB in human and mouse cell lysates. Western blot analysis using Enhancer of polycomb homolog 2 Antibody on 30 ug of reducing whole-cell lysate per lane. Lanes 1-5 contain human HEL, K562, SH-SY5Y and RT4 cells, followed by mouse Neuro-2a cells. Samples were resolved on a 10% SDS-PAGE gel, transferred to nitrocellulose and probed with 0.5 ug/ml primary antibody overnight at 4oC. HRP-conjugated goat anti-rabbit IgG and chemiluminescent detection revealed a band near 91 kDa in each lane, consistent with the calculated EPC2 molecular weight of approximately 91 kDa.