EPB41L5 Antibody / Epithelial-Mesenchymal Transition Protein Antibody (FY13498)

EPB41L5 Antibody Human Colon Cancer Cell IF. Immunofluorescent staining of CACO-2 cells using EPB41L5 antibody demonstrated predominantly cytoplasmic fluorescence (green) with enrichment along the cell periphery, consistent with the role of EPB41L5 in epithelial-mesenchymal transition, cell adhesion remodeling, and cytoskeletal organization. Cells were treated with enzyme antigen retrieval reagent for 15 minutes, blocked with 10% goat serum, and incubated with the primary antibody (5 ug/ml) overnight at 4°C. Fluoro488-conjugated goat anti-rabbit IgG was used as the secondary antibody, and nuclei were counterstained with DAPI (blue). This EPB41L5 Antibody, also called an Epithelial-Mesenchymal Transition Protein Antibody, is useful for investigating epithelial-mesenchymal transition, cell adhesion dynamics, tumor invasion, and metastatic progression.

EPB41L5 Antibody Human, Rat and Mouse WB. Western blot testing of human MCF-7 (lane 1), Caco-2 (lane 2), HepG2 (lane 3), U251 (lane 4), rat kidney (lane 5), rat eye (lane 6), mouse kidney (lane 7), and mouse eye (lane 8) lysates using EPB41L5 antibody detected a major band at approximately 58 kDa, consistent with one reported isoform of EPB41L5. Although an additional isoform of approximately 82 kDa has been described, the lower molecular weight form predominated under these experimental conditions. This EPB41L5 Antibody, also called an Epithelial-Mesenchymal Transition Protein Antibody, is useful for investigating epithelial-mesenchymal transition, cell adhesion remodeling, cytoskeletal organization, and cancer metastasis.

EPB41L5 Antibody Human Melanoma IHC. Immunohistochemical staining of FFPE human melanoma tissue using EPB41L5 antibody demonstrated predominantly membranous and cytoplasmic staining in tumor cells, consistent with the role of EPB41L5 in epithelial-mesenchymal transition, cell adhesion remodeling, and cell migration. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen. This EPB41L5 Antibody, also called an Epithelial-Mesenchymal Transition Protein Antibody, is useful for investigating epithelial-mesenchymal transition, cell adhesion remodeling, tumor invasion, and cancer metastasis.

EPB41L5 Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue using EPB41L5 antibody demonstrated predominantly membranous and cytoplasmic staining in malignant cells, consistent with the role of EPB41L5 in epithelial-mesenchymal transition, cell adhesion remodeling, and tumor cell migration. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen. This EPB41L5 Antibody, also called an Epithelial-Mesenchymal Transition Protein Antibody, is useful for investigating epithelial-mesenchymal transition, cell adhesion remodeling, tumor invasion, and metastatic progression.