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Home >> Antibodies >> ENT1 Antibody

ENT1 Antibody (F54342)

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  Catalog No Formulation Size Price (USD)  
Image F54342-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54342-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human 1) brain, 2) breast and 3) placenta lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
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Western blot testing of human 1) brain, 2) breast and 3) placenta lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
Western blot testing of human 1) heart, 2) breast and 3) placenta lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
Western blot testing of mouse 1) heart and 2) liver lysate with ENT1 antibody. Predicted molecular weight ~50 kDa.
IHC testing of FFPE human brain tissue with ENT1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with ENT1 antibody; Blue=isotype control, Green= ENT1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q99808
Applications Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This ENT1 antibody is available for research use only.
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Description

ENT1, or Equilibrative nucleoside transporter 1, is a member of the equilibrative nucleoside transporter family. It is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ENT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 402-431 from the human protein was used as the immunogen for the ENT1 antibody.

Storage

Aliquot the ENT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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