ENPP4 Antibody / Bis(5'-adenosyl)-triphosphatase ENPP4 Antibody (FY13645)

ENPP4 Antibody by WB in human cell lines. Western blot analysis using Bis(5'-adenosyl)-triphosphatase ENPP4 Antibody detects a prominent band at approximately 47-50 kDa in three human cell lysates. Lane 1 contains HEK293T cells, lane 2 contains U251 cells, and lane 3 contains Jurkat cells. The principal band migrates at a similar apparent molecular weight across the samples. An additional lower band is visible in the Jurkat lane, but its identity is not established. The figure includes molecular weight markers to support assessment of apparent band size.

ENPP4 Antibody by IHC in human tonsil tissue. Immunohistochemistry analysis of ENPP4 expression in a paraffin-embedded section of human tonsil tissue using ENPP4 Antibody. Heat-mediated antigen retrieval was performed with EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated overnight at 4oC with the primary antibody at 2 ug/ml. A peroxidase-conjugated goat anti-rabbit IgG secondary antibody was applied, and immunoreactivity was visualized using DAB chromogen.

ENPP4 Antibody by FCM in fixed HEK293T cells. Flow cytometry analysis using Bis(5'-adenosyl)-triphosphatase ENPP4 Antibody shows a rightward shift in fluorescence for the antibody-stained population (blue) compared with the isotype control (green) and unstained blank (red). The histogram illustrates ENPP4-associated immunoreactivity in fixed HEK293T cells. This result should not be interpreted as demonstrating surface staining of live cells.