ENOSF1 Antibody / Mitochondrial enolase superfamily member 1 Antibody (FY13592)

ENOSF1 Antibody by IHC in human thyroid carcinoma. Immunohistochemistry of paraffin-embedded human thyroid carcinoma using Mitochondrial enolase superfamily member 1 Antibody shows brown staining within tumor epithelial cells surrounding glandular spaces. Tissue sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection. Hematoxylin provides the blue nuclear counterstain.

ENOSF1 Antibody by WB in human and rodent samples. Western blot analysis using Mitochondrial enolase superfamily member 1 Antibody detects an immunoreactive band near 50 kDa in human HeLa (lane 1), K562 (lane 2), MCF-7 (lane 3), RT4 (lane 4), rat kidney (lane 5), and mouse kidney (lane 6). The calculated molecular weight is approximately 50 kDa. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.

ENOSF1 Antibody by IP in RT4 cells. Immunoprecipitation using Mitochondrial enolase superfamily member 1 Antibody was evaluated by Western blotting. Lane 1 contains RT4 input lysate (30 ug), lane 2 contains the rabbit IgG immunoprecipitation control, and lane 3 contains material precipitated from 500 ug RT4 lysate with 2 ug antibody. A band near 50 kDa is enriched in the specific immunoprecipitate relative to the control, consistent with the expected molecular weight of ENOSF1. Immunoprecipitated proteins were transferred to a membrane, detected with primary antibody at 0.5 ug/mL, and visualized by HRP-based chemiluminescence.

ENOSF1 Antibody by FACS in K562 cells. Flow cytometry using Mitochondrial enolase superfamily member 1 Antibody measures intracellular ENOSF1 staining in fixed, permeabilized K562 cells. The antibody-stained population is shown in blue, with rabbit IgG isotype control in green and unstained control in red. Cells were fixed with 4% paraformaldehyde, permeabilized, and blocked with 10% normal goat serum. Primary antibody was applied at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by Fluoro488-conjugated secondary antibody. The experiment does not assess cell-surface binding on live cells.