Enolase 1 Antibody / Alpha Enolase / ENO1 (RQ5668)

Description

Enolase 1 is a glycolytic enzyme that catalyzes the reversible interconversion of 2-phosphoglycerate and phosphoenolpyruvate, supporting ATP production and metabolic adaptability. In addition to its cytosolic role, Enolase 1 can be detected at the cell surface and in the nucleus, where it contributes to plasminogen binding and broader cell state regulation.

Enolase 1 abundance and localization respond to metabolic cues, hypoxia, and differentiation status, making it a versatile marker in studies of bioenergetics and cellular remodeling. Its participation in core energy pathways and context-dependent “moonlighting” functions supports research across cell biology and translational workflows.

The Enolase 1 antibody enables specific detection of endogenous ENO1 in applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA. Researchers use the Enolase 1 antibody to quantify protein levels, track subcellular distribution, and evaluate responses to genetic or pharmacologic perturbation. With high specificity and consistent performance, the Enolase 1 antibody supports rigorous analysis of glycolysis, metabolic rewiring, and cell state transitions.

Application Notes

Optimal dilution of the Enolase 1 antibody should be determined by the researcher.

Immunogen

Amino acids DDPSRYISPDQLADLYKSFIKD from the human protein were used as the immunogen for the Enolase 1 antibody.

Storage

After reconstitution, the Enolase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.