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Home >> Antibodies >> ENO2 Antibody / Neuron Specific Enolase / Gamma Enolase

ENO2 Antibody / Neuron Specific Enolase / Gamma Enolase [clone rENO/8857] (V4226)

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  Catalog No Formulation Size Price (USD)  
Image V4226-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4226-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4226SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated Recmousemono
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ENO2 Antibody Pancreas IHC. Immunohistochemistry staining of FFPE human pancreas tissue with ENO2 antibody (clone rENO/8857). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rENO/8857
Purity Protein A/G affinity
UniProt P09104
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This ENO2 antibody is available for research use only.
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Description

ENO2 Antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It is usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

For detection of neuron-specific enolase (NSE), also known as gamma enolase, across tissue types, see our NSE antibody.

Application Notes

Optimal dilution of the ENO2 antibody should be determined by the researcher.

Immunogen

A recombinant fragment of human NSE gamma (within amino acids 416-433) was used as the immunogen for the ENO2 antibody.

Storage

Aliquot the ENO2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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