EMSY Antibody / BRCA2-interacting transcriptional repressor EMSY Antibody (FY13564)

EMSY Antibody by IF in U2OS cells. Immunofluorescence analysis using BRCA2-interacting transcriptional repressor EMSY Antibody shows predominantly nuclear green staining in U2OS cells, consistent with the known nuclear localization of this chromatin-associated protein. Alpha-tubulin is counterstained red to outline the cytoskeleton. Cells were incubated with primary antibody and visualized with fluorescently labeled secondary antibodies. The merged image demonstrates green nuclear signal and red cytoplasmic microtubules.

EMSY Antibody by IHC in human prostatic hyperplasia. Immunohistochemistry of formalin-fixed, paraffin-embedded human prostatic hyperplasia using BRCA2-interacting transcriptional repressor EMSY Antibody shows prominent brown epithelial staining within glandular structures. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum and incubation with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen were used to visualize immunoreactivity, with blue nuclear counterstaining.

EMSY Antibody by WB in human MCF-7 cells. Western blot analysis using BRCA2-interacting transcriptional repressor EMSY Antibody detects a prominent band near 160 kDa in MCF-7 whole-cell lysate, above the calculated molecular weight of approximately 141.5 kDa. Independent antibody characterization has also reported EMSY migration above its calculated mass, but the basis for the shift in this sample has not been established. The lysate was resolved by 8% SDS-PAGE and transferred to a membrane for immunodetection. The observed high-molecular-weight signal is labeled EMSY in the blot; a lower band is also visible but has not been identified.

EMSY Antibody by FACS in THP-1 cells. Intracellular flow cytometry using BRCA2-interacting transcriptional repressor EMSY Antibody shows a right-shifted blue fluorescence distribution compared with the green rabbit IgG isotype control and red unstained control. Cells were fixed with 4% paraformaldehyde, permeabilized and blocked with 10% goat serum before staining with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC. Fluoro488-conjugated secondary antibody was applied for 30 minutes before acquisition.