EML1 Antibody / EMAP like 1 Antibody (FY13614)

EML1 Antibody by IHC in rat brain. Immunohistochemistry of paraffin-embedded rat brain using Echinoderm microtubule-associated protein-like 1 Antibody demonstrates distinct brown staining in scattered neuronal cell bodies against a lightly stained neuropil. Heat-mediated antigen retrieval was performed with EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen produced the brown reaction product, while hematoxylin counterstained cell nuclei blue.

EML1 Antibody by WB in rodent brain. Western blot analysis using Echinoderm microtubule-associated protein-like 1 Antibody detects a prominent band consistent with the expected molecular weight of approximately 90 kDa in rat brain (lane 1) and mouse brain (lane 2) tissue lysates. Reduced samples containing 30 ug total protein per lane were separated under reducing conditions by 10% SDS-PAGE and transferred to nitrocellulose. Following blocking with 5% nonfat milk, membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Bound antibody was visualized with HRP-conjugated secondary antibody and chemiluminescent substrate.

EML1 Antibody by IHC in human brain. Immunohistochemistry of paraffin-embedded human brain using Echinoderm microtubule-associated protein-like 1 Antibody demonstrates scattered brown-stained cellular profiles and processes throughout the neuropil. Heat-mediated antigen retrieval was performed with EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen produced the brown reaction product, while hematoxylin counterstained cell nuclei blue.

EML1 Antibody by IHC in mouse brain. Immunohistochemistry of paraffin-embedded mouse brain using Echinoderm microtubule-associated protein-like 1 Antibody demonstrates brown staining of scattered neurons and cell processes in the surrounding brain tissue. Heat-mediated antigen retrieval was performed with EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen produced the brown reaction product, while hematoxylin counterstained cell nuclei blue.