EMILIN2 Antibody / EMILIN-2 Antibody (FY13613)

EMILIN2 Antibody by IHC in human lung carcinoma. Immunohistochemistry of paraffin-embedded human lung carcinoma using EMILIN-2 Antibody demonstrates strong brown extracellular and pericellular staining among tumor and stromal cells. Heat-mediated antigen retrieval was performed with EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen produced the brown reaction product, while hematoxylin counterstained cell nuclei blue.

EMILIN2 Antibody by WB in human THP-1 cells. Western blot analysis using EMILIN-2 Antibody detects a prominent band consistent with the expected molecular weight of approximately 116 kDa in human THP-1 whole-cell lysate (lane 1). The band migrates near 125 kDa. Reduced samples containing 30 ug total protein per lane were separated under reducing conditions by 8% SDS-PAGE and transferred to nitrocellulose. Following blocking with 5% nonfat milk, membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Bound antibody was visualized with HRP-conjugated secondary antibody and chemiluminescent substrate.

EMILIN2 Antibody by IHC in human endometrial carcinoma. Immunohistochemistry of paraffin-embedded human endometrial carcinoma using EMILIN-2 Antibody demonstrates brown staining concentrated around malignant glandular structures and associated extracellular matrix. Heat-mediated antigen retrieval was performed with EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen produced the brown reaction product, while hematoxylin counterstained cell nuclei blue.