EMI1 Antibody / Cell Cycle Checkpoint Regulator Antibody [clone EMI1/1176] (V2527)

Description

F-box only protein 5 (FBXO5), also known as Early mitotic inhibitor 1 (EMI1), is a cell cycle regulatory protein involved in control of S phase progression, mitotic entry, and APC/C-associated checkpoint signaling. The EMI1 Antibody / Cell Cycle Checkpoint Regulator Antibody clone EMI1/1176 is designed for highly selective detection of EMI1 expression in studies involving cell cycle progression, mitotic regulation, and replication-associated signaling pathways.

EMI1 is encoded by the FBXO5 gene on chromosome 6q25 and functions primarily within the nucleus and cytoplasmic cell cycle regulatory compartments. The protein acts as an inhibitor of the anaphase-promoting complex/cyclosome (APC/C), thereby preventing premature degradation of cyclins and other proteins required for orderly cell cycle progression. Through this regulatory role, EMI1 contributes to maintenance of genomic stability and proper timing of mitotic transitions.

Expression of EMI1 is tightly coordinated with proliferative activity and is frequently elevated during actively cycling cellular states. The protein accumulates during S and G2 phases and is subsequently degraded during mitosis through SCF-betaTrCP-mediated ubiquitination pathways. Dysregulation of EMI1 expression has been associated with aberrant proliferation, chromosomal instability, replication stress responses, and oncogenic transformation across multiple tumor types.

EMI1 also participates in broader networks governing DNA replication licensing and checkpoint-associated signaling. By modulating APC/C activity, EMI1 helps preserve the stability of proteins required for DNA synthesis and mitotic fidelity. Loss of proper EMI1 regulation may contribute to rereplication events, mitotic defects, and altered cellular sensitivity to genotoxic stress. These biologic functions have generated continued interest in EMI1 across cancer biology, cell cycle research, and therapeutic response studies.

The clone EMI1/1176 monoclonal antibody is further differentiated by protein microarray specificity validation performed against a HuProt(TM) array containing more than 19,000 full-length human proteins. This large-scale specificity screening approach helps evaluate potential cross-reactivity across the human proteome and is particularly valuable for cell cycle-associated proteins that share conserved regulatory domains and overlapping molecular interactions. The resulting specificity profile supports use of this EMI1 antibody in studies where selective target recognition and reduced off-target binding are important experimental considerations.

Immunohistochemical and western blot analyses using EMI1 antibodies commonly demonstrate expression patterns associated with proliferative cellular compartments and actively cycling tumor populations. EMI1 continues to serve as an important marker within studies involving mitotic checkpoint regulation, APC/C signaling, replication stress biology, and tumor-associated proliferative pathways.

Additional antibodies involved in mitotic regulation, DNA replication control, and proliferative checkpoint signaling can be explored within our Signal Transduction Antibodies collection.

Application Notes

Optimal dilution of the EMI1 Antibody / Cell Cycle Checkpoint Regulator Antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.

Storage

Store the EMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

EMI1 antibody, FBXO5 antibody, Early mitotic inhibitor 1 antibody, Cell cycle checkpoint regulator antibody, APC/C regulator antibody