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Home >> Antibodies >> EMI-1 Antibody

EMI-1 Antibody [clone FBXP5] (V7090)

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  Catalog No Formulation Size Price (USD)  
Image V7090-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7090-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7090SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7090IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Western blot testing of human 1) HeLa and 2) 293 lysate with EMI-1 antibody. Predicted molecular weight: 50/45 kDa (isoforms 1/2).
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Western blot testing of human 1) HeLa and 2) 293 lysate with EMI-1 antibody. Predicted molecular weight: 50/45 kDa (isoforms 1/2).
IHC testing of FFPE human ovarian carcinoma and EMI-1 antibody (clone FBXP5). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name FBXP5
Purity Protein G affinity chromatography
UniProt Q9UKT4
Localization Nuclear
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This EMI-1 antibody is available for research use only.
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Description

Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Application Notes

Optimal dilution of the EMI-1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Storage

Store the EMI-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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