EMG1 Antibody / EMG1 N1-specific pseudouridine methyltransferase Antibody (FY13608)

EMG1 Antibody by IF in HeLa cells. Immunofluorescence staining of HeLa cells using EMG1 N1-specific pseudouridine methyltransferase Antibody reveals prominent green nuclear and nucleolar-region staining with red cytoplasmic alpha-tubulin. Target immunoreactivity is shown in green, with alpha-tubulin labeling in red. The cells underwent enzyme-based antigen retrieval, blocking with 10% goat serum, and overnight primary antibody incubation at 5 ug/mL at 4oC. Fluorescent secondary antibodies were applied at 1:500 before microscopy.

EMG1 Antibody by IHC in human lung carcinoma. Immunohistochemistry of paraffin-embedded human lung carcinoma using EMG1 N1-specific pseudouridine methyltransferase Antibody shows brown immunoreactivity in tumor epithelial cells, with variable nuclear and cytoplasmic distribution. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Immunoreactivity was visualized with an HRP-conjugated secondary antibody and DAB chromogen; hematoxylin counterstaining identifies tissue nuclei.

EMG1 Antibody by WB in human and rodent samples. Western blot analysis using EMG1 N1-specific pseudouridine methyltransferase Antibody detects a prominent band consistent with the expected molecular weight of approximately 27 kDa in human 293T (lane 1), HeLa (lane 2), Caco-2 (lane 3), rat thymus (lane 4), RH35 (lane 5), mouse thymus (lane 6), and NIH/3T3 (lane 7). Reduced lysates containing 30 ug protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. Following blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and chemiluminescence.