EMA Antibody MUC1/845 | V2371 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2371-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2371-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2371SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2371IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 / EMA antibody (MUC1/845).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).

SDS-PAGE Analysis of Purified, BSA-Free EMA Antibody (clone MUC1/845). Confirmation of Integrity and Purity of the Antibody.

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	MUC1/845
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	4582
Localization	Cytoplasmic and cell surface
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This EMA antibody is available for research use only.
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Description

This antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen). The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EMA antibody to be titered up or down for optimal performance.

1. HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 10-20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.