ELF2 Antibody / E74-like ETS transcription factor 2 (FY13458)

Flow cytometry analysis of ELF2 using ELF2 antibody. ELF2 expression was examined in human A2780 cells following fixation with 4% paraformaldehyde and permeabilization to enable intracellular staining. Cells were incubated with ELF2 antibody and detected using a fluorescent secondary antibody (blue). An isotype control stained under identical conditions is shown in green, and an unstained control is shown in red. The rightward fluorescence shift observed with ELF2 antibody staining indicates specific intracellular detection of ELF2.

Western blot analysis of ELF2 using ELF2 antibody. ELF2 expression was examined in human K562 and human Reh whole cell lysates. A primary band is detected at approximately 57 kDa, which migrates slightly below the predicted molecular weight of approximately 64 kDa based on the ELF2 amino acid sequence. This modest downward shift is consistent with reported electrophoretic behavior of some ETS family transcription factors and may reflect SDS-PAGE migration properties, alternative isoform usage, or differential post-translational processing rather than protein truncation.