ELAC2 Antibody / Zinc phosphodiesterase ELAC protein 2 Antibody (FY13561)

ELAC2 Antibody by IF in A549 cells. Immunofluorescence staining of A549 cells using Zinc phosphodiesterase ELAC protein 2 Antibody reveals predominantly nuclear green staining. Published studies document both nuclear and mitochondrial ELAC2 localization; the staining pattern shown here has not been assigned to a specific translation product. Target immunoreactivity is shown in green, with alpha-tubulin in red. Cells were treated with enzyme antigen retrieval reagent, blocked with 10% goat serum and incubated with primary antibody at 5 ug/mL overnight at 4oC. Fluorescent secondary antibodies were used for microscopy.

ELAC2 Antibody by IHC in mouse testis. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse testis using Zinc phosphodiesterase ELAC protein 2 Antibody shows brown immunoreactivity within seminiferous tubules, including prominent nuclear staining in subsets of germ cells. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum and incubation with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen were used to visualize immunoreactivity, with blue nuclear counterstaining.

ELAC2 Antibody by WB in human cell lines. Western blot analysis using Zinc phosphodiesterase ELAC protein 2 Antibody detects a band near 92 kDa in HeLa (lane 1), HepG2 (lane 2), and MCF-7 (lane 3) whole-cell lysates. The observed migration agrees with the approximately 92 kDa expected molecular weight of ELAC2. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.

ELAC2 Antibody by IHC in human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded human brain using Zinc phosphodiesterase ELAC protein 2 Antibody shows widespread brown staining across neuropil and subsets of cell bodies. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum and incubation with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen were used to visualize immunoreactivity, with blue nuclear counterstaining.

ELAC2 Antibody by IP in HepG2 lysates. Immunoprecipitation using Zinc phosphodiesterase ELAC protein 2 Antibody enriches a band near 92 kDa from HepG2 whole-cell lysate. Lane 1 contains 30 ug input lysate; lane 2 is the rabbit IgG control immunoprecipitation; lane 3 contains immunoprecipitated material using 2 ug primary antibody and 500 ug lysate. Following transfer, ELAC2 was detected by Western blot with primary antibody at 0.5 ug/mL and an HRP-conjugated secondary antibody. A prominent immunoglobulin heavy-chain signal is visible in the immunoprecipitated lane.