EIF4G1 Antibody (RQ5515)

EIF4G1 Antibody Human U2OS Cell IF. Immunofluorescent staining of U2OS cells using EIF4G1 antibody demonstrated prominent cytoplasmic fluorescence (green), consistent with the localization of EIF4G1 as a translation initiation factor involved in mRNA translation and protein synthesis. Cells were treated with enzyme antigen retrieval reagent for 15 minutes, blocked with 10% goat serum, and incubated with the primary antibody (2 ug/ml) overnight at 4°C. DyLight 488-conjugated goat anti-rabbit IgG was used as the secondary antibody, and nuclei were counterstained with DAPI (blue). This EIF4G1 Antibody, also called a Translation Initiation Factor Antibody, is useful for investigating translational control, protein synthesis, and subcellular localization of translation initiation complexes.

EIF4G1 Antibody Human, Rat and Mouse WB. Western blot testing of human K562 (lane 1), CACO-2 (lane 2), A549 (lane 3), rat brain (lane 4), and mouse brain (lane 5) lysates using EIF4G1 antibody detected a prominent band at approximately 250 kDa. Although the calculated molecular weight of EIF4G1 is approximately 175 kDa, the protein commonly migrates at a substantially higher apparent molecular weight because of its large size, extended structure, and post-translational modifications. This EIF4G1 Antibody, also called a Translation Initiation Factor Antibody, is useful for investigating translational control, protein synthesis, and mRNA translation.

EIF4G1 Antibody Human Breast Cancer IHC. Immunohistochemical staining of FFPE human breast cancer tissue using EIF4G1 antibody demonstrated strong cytoplasmic staining in malignant epithelial cells, consistent with the role of EIF4G1 as a translation initiation factor that regulates protein synthesis. Heat-induced epitope retrieval was performed in pH6 citrate buffer for 20 minutes prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (1 ug/ml) overnight at 4°C, followed by a biotinylated goat anti-rabbit IgG secondary antibody and Streptavidin-Biotin Complex (SABC) detection with DAB as the chromogen. This EIF4G1 Antibody, also called a Translation Initiation Factor Antibody, is useful for investigating translational control, protein synthesis, oncogenic signaling, and breast cancer biology.

EIF4G1 Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue using EIF4G1 antibody demonstrated strong cytoplasmic staining in malignant cells, consistent with the role of EIF4G1 as a translation initiation factor that regulates protein synthesis. Heat-induced epitope retrieval was performed in pH6 citrate buffer for 20 minutes prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (1 ug/ml) overnight at 4°C, followed by a biotinylated goat anti-rabbit IgG secondary antibody and Streptavidin-Biotin Complex (SABC) detection with DAB as the chromogen. This EIF4G1 Antibody, also called a Translation Initiation Factor Antibody, is useful for investigating translational control, mRNA translation, oncogenic signaling, and lung cancer biology.

EIF4G1 Antibody Human Intestinal Cancer IHC. Immunohistochemical staining of FFPE human intestinal cancer tissue using EIF4G1 antibody demonstrated predominantly cytoplasmic staining in malignant epithelial cells, consistent with the role of EIF4G1 as a translation initiation factor involved in protein synthesis. Heat-induced epitope retrieval was performed in pH6 citrate buffer for 20 minutes prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (1 ug/ml) overnight at 4°C, followed by a biotinylated goat anti-rabbit IgG secondary antibody and Streptavidin-Biotin Complex (SABC) detection with DAB as the chromogen. This EIF4G1 Antibody, also called a Translation Initiation Factor Antibody, is useful for investigating mRNA translation, protein synthesis, oncogenic signaling, and cancer biology.

EIF4G1 Antibody Mouse Intestine IHC. Immunohistochemical staining of mouse intestine tissue using EIF4G1 antibody demonstrated prominent cytoplasmic staining of intestinal epithelial cells, consistent with the role of EIF4G1 as a translation initiation factor that supports active protein synthesis in rapidly renewing tissues. Heat-induced epitope retrieval was performed in pH6 citrate buffer for 20 minutes prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (1 ug/ml) overnight at 4°C, followed by a biotinylated goat anti-rabbit IgG secondary antibody and Streptavidin-Biotin Complex (SABC) detection with DAB as the chromogen. This EIF4G1 Antibody, also called a Translation Initiation Factor Antibody, is useful for investigating mRNA translation, protein synthesis, intestinal biology, and translational control.

EIF4G1 Antibody Rat Intestine IHC. Immunohistochemical staining of rat intestine tissue using EIF4G1 antibody demonstrated prominent cytoplasmic staining of intestinal epithelial cells, particularly within the mucosal lining and crypt epithelium, consistent with the role of EIF4G1 as a translation initiation factor supporting active protein synthesis. Heat-induced epitope retrieval was performed in pH6 citrate buffer for 20 minutes prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (1 ug/ml) overnight at 4°C, followed by a biotinylated goat anti-rabbit IgG secondary antibody and Streptavidin-Biotin Complex (SABC) detection with DAB as the chromogen. This EIF4G1 Antibody, also called a Translation Initiation Factor Antibody, is useful for investigating mRNA translation, protein synthesis, intestinal physiology, and translational control.

EIF4G1 Antibody Human Liver Cancer Cell FACS. Flow cytometry analysis of fixed and permeabilized human HepG2 cells using EIF4G1 antibody demonstrated a marked rightward shift of the stained population (blue) compared with the isotype control (green), indicating specific intracellular detection of EIF4G1. Cells were fixed with 4% paraformaldehyde, permeabilized, blocked with 10% normal goat serum, and incubated with the primary antibody (1 ug/1×10^6 cells) followed by DyLight 488-conjugated goat anti-rabbit IgG. The red histogram represents the unstained blank control. This EIF4G1 Antibody, also called a Translation Initiation Factor Antibody, is useful for investigating translational control, protein synthesis, and EIF4G1 expression by flow cytometry.