- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
EIF4A3 Antibody / Eukaryotic initiation factor 4A-III (FY13354)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY13354 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
EIF4A3 antibody detects Eukaryotic initiation factor 4A-III, an RNA helicase encoded by the EIF4A3 gene located on chromosome 17q25.3. EIF4A3 is a nuclear and cytoplasmic ATP-dependent RNA helicase belonging to the DEAD-box family and serves as a core component of the exon junction complex (EJC). The protein is essential for mRNA splicing, export, surveillance, and translation regulation. EIF4A3 is ubiquitously expressed but shows particularly high levels in brain, testis, and proliferating tissues where mRNA metabolism is active.
EIF4A3 functions as an mRNA quality control factor by binding to spliced mRNAs upstream of exon-exon junctions as part of the EJC, together with MAGOH, RBM8A (Y14), and MLN51. This complex regulates nonsense-mediated mRNA decay and translation efficiency, ensuring that defective transcripts are degraded. EIF4A3 also acts independently as an RNA helicase that unwinds RNA secondary structures to facilitate ribosome scanning during translation initiation. Co-localization studies show EIF4A3 concentrated in nuclear speckles and cytoplasmic ribonucleoprotein granules, reflecting its dual role in RNA processing and translation.
Structurally, EIF4A3 contains two RecA-like domains forming an ATP-binding cleft and conserved motifs such as the DEAD (Asp-Glu-Ala-Asp) box essential for helicase activity. It belongs to the DEAD-box helicase subfamily of eukaryotic initiation factors, which also includes EIF4A1 and EIF4A2. Unlike these cytoplasmic isoforms, EIF4A3 primarily functions in the nucleus as part of the EJC, anchoring the complex to mRNA. Known interacting partners include MAGOH, Y14, UPF1, and CASC3.
Functionally, EIF4A3 is indispensable for mRNA stability, nonsense-mediated decay, and post-transcriptional gene regulation. It coordinates RNA splicing and surveillance with translation, maintaining proteome integrity. In neurons, EIF4A3 regulates synaptic mRNA localization and local protein synthesis critical for plasticity. During embryonic development, EIF4A3 contributes to neural differentiation and morphogenesis by regulating gene expression at the RNA level.
Mutations or depletion of EIF4A3 disrupts RNA surveillance, leading to aberrant transcript accumulation and developmental defects. Loss-of-function variants cause Richieri-Costa-Pereira syndrome, characterized by craniofacial and limb malformations. Dysregulation of EIF4A3 expression has also been associated with tumor progression, as enhanced RNA surveillance supports oncogenic growth. Pathway associations include mRNA splicing, nonsense-mediated decay, and translational control.
Immunohistochemical staining using EIF4A3 antibody demonstrates nuclear and cytoplasmic localization in neurons, epithelial cells, and germ cells. The EIF4A3 antibody from NSJ Bioreagents is a useful reagent for research into RNA metabolism, splicing regulation, and translational control mechanisms.
Optimal dilution of the EIF4A3 antibody should be determined by the researcher.
E.coli-derived human EIF4A3 recombinant protein (Position: R14-I411) was used as the immunogen for the EIF4A3 antibody.
After reconstitution, the EIF4A3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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