EIF3M Antibody / Eukaryotic translation initiation factor 3 subunit M Antibody (FY13589)

EIF3M Antibody by IHC in human endometrial carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human endometrial carcinoma using Eukaryotic translation initiation factor 3 subunit M Antibody shows strong brown immunoreactivity in tumor epithelial regions, with blue nuclear counterstaining. Heat-induced antigen retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen generated brown immunoreactivity, with hematoxylin counterstaining highlighting nuclei.

EIF3M Antibody by WB in human cells and rodent tissues. Western blot analysis using Eukaryotic translation initiation factor 3 subunit M Antibody detects a prominent band near 38 kDa, compared with the calculated molecular weight of approximately 42 kDa. Lanes 1-4 contain HeLa, HepG2, MCF-7 and A549 cell lysates; lanes 5-8 contain rat brain, rat stomach, mouse brain and mouse stomach lysates. Reduced lysates containing 30 ug total protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection employed an HRP-conjugated anti-rabbit secondary antibody and chemiluminescent substrate.

EIF3M Antibody by IHC in mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse brain using Eukaryotic translation initiation factor 3 subunit M Antibody shows scattered brown cellular staining within brain parenchyma. Heat-induced antigen retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen generated brown immunoreactivity, with hematoxylin counterstaining highlighting nuclei.

EIF3M Antibody by IHC in rat brain. Immunohistochemistry of formalin-fixed, paraffin-embedded rat brain using Eukaryotic translation initiation factor 3 subunit M Antibody shows brown staining in scattered cells across the examined brain section. Heat-induced antigen retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen generated brown immunoreactivity, with hematoxylin counterstaining highlighting nuclei.