EIF3K Antibody / Eukaryotic translation initiation factor 3 subunit K Antibody (FY13599)

EIF3K Antibody by IF in A549 cells. Immunofluorescence staining using Eukaryotic translation initiation factor 3 subunit K Antibody shows diffuse green cytoplasmic fluorescence surrounding DAPI-positive nuclei, with some green signal overlapping nuclear regions. Nuclei are counterstained blue with DAPI. Cells underwent enzyme-mediated antigen retrieval for 15 minutes and blocking with 10% goat serum. Primary antibody was applied at 5 ug/mL overnight at 4oC, followed by a Fluoro488-conjugated secondary antibody at 1:500 for 30 minutes at 37oC. The observed staining distribution is described without assuming organelle-specific colocalization.

EIF3K Antibody by IHC in human liver carcinoma. Immunohistochemistry of paraffin-embedded human liver carcinoma using Eukaryotic translation initiation factor 3 subunit K Antibody reveals widespread brown cytoplasmic staining in malignant epithelial cells. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB chromogen. Hematoxylin counterstains the nuclei blue.

EIF3K Antibody by WB in human and rodent samples. Western blot analysis using Eukaryotic translation initiation factor 3 subunit K Antibody detects a prominent immunoreactive band near 25 kDa in human 293T (lane 1), HeLa (lane 2), A549 (lane 3), RT4 (lane 4), rat brain (lane 5), and mouse brain (lane 6). The calculated molecular weight is approximately 25 kDa. Reduced samples containing 30 ug protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody at 1:5000 and a chemiluminescent substrate. The band positions are described relative to the molecular weight standards in the blot.

EIF3K Antibody by IP in HeLa cells. Immunoprecipitation using Eukaryotic translation initiation factor 3 subunit K Antibody was evaluated by Western blot. Lane 1 contains 30 ug HeLa input lysate, lane 2 contains the rabbit IgG control immunoprecipitate, and lane 3 contains material precipitated using 2 ug antibody and 500 ug HeLa lysate. A band near 25 kDa appears in the input and specific immunoprecipitate, consistent with the calculated EIF3K molecular weight. Immunoglobulin heavy chain is visible around 50 kDa in the immunoprecipitation lanes. The detection antibody was used at 0.5 ug/mL with HRP-conjugated secondary antibody and chemiluminescence.

EIF3K Antibody by FACS in RT4 cells. Flow cytometry using Eukaryotic translation initiation factor 3 subunit K Antibody detects intracellular staining in fixed, permeabilized RT4 cells. The antibody-stained population is blue and shifted to the right of the green rabbit IgG isotype control and red unstained control. Cells were fixed with 4% paraformaldehyde, permeabilized, and blocked with 10% normal goat serum. Primary antibody was used at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by Fluoro488-conjugated secondary antibody. This assay does not assess live-cell surface staining.