EIF3I Antibody / Eukaryotic translation initiation factor 3 subunit I Antibody (FY13573)

EIF3I Antibody by WB in human and rodent lysates. Western blot analysis using Eukaryotic translation initiation factor 3 subunit I Antibody detects a prominent immunoreactive band near 37 kDa in SH-SY5Y (lane 1), Jurkat (lane 2), rat brain (lane 3), rat RH35 (lane 4), mouse brain (lane 5), and mouse NIH/3T3 (lane 6). The calculated molecular weight is approximately 36.5 kDa. Samples were analyzed under reducing conditions with 30 ug protein per lane using 10% SDS-PAGE, followed by transfer to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and chemiluminescent substrate were used for visualization.

EIF3I Antibody by IHC in human liver carcinoma. Immunohistochemistry of paraffin-embedded human liver carcinoma using Eukaryotic translation initiation factor 3 subunit I Antibody demonstrates brown cytoplasmic staining in tumor cells, with variation in intensity between malignant cell clusters, with hematoxylin-counterstained nuclei appearing blue. Sections were subjected to heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and blocked with 10% goat serum. The primary antibody was incubated at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection.