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Home >> Antibodies >> EIF3B Antibody

EIF3B Antibody (R31139)

  Catalog No Formulation Size Price (USD)  
Image R31139 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) A375, 2) K562 and 3) MCF7 cell lysate with EIF3B antibody. Expected molecular weight ~116 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P55884
Applications Western blot : 0.5-1ug/ml
Limitations This EIF3B antibody is available for research use only.
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Description

Eukaryotic Translation Initiation Factor 3, Subunit B, also called PRT1 or EIF3-p116, is a protein that in humans is encoded by the EIF3B gene. By searching an EST database, Methot et al.(1997) identified a cDNA encoding a human Prt1 homolog. Asano et al.(1997) demonstrated that the 115-kD component of HeLa cell eIF3 is actually composed of two proteins, p116 (EIF3B) and an unrelated 110-kD protein.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the EIF3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Eukaryotic Translation Initiation Factor 3, Subunit B (EDFRKYRKMAQELYMEQKNER) was used as the immunogen for this Eif3b antibody.

Storage

After reconstitution, the EIF3B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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