EIF2S3 Antibody / Eukaryotic translation initiation factor 2 subunit 3 Antibody (FY13619)

EIF2S3 Antibody by FACS in K562 cells. Flow cytometry using Eukaryotic translation initiation factor 2 subunit 3 Antibody shows a rightward fluorescence shift in fixed, permeabilized K562 cells stained with primary antibody (blue histogram), compared with the rabbit IgG isotype control (green) and unstained blank control (red). Cells were fixed in 4% paraformaldehyde, permeabilized, and blocked with 10% normal goat serum. Primary antibody was applied at 1 ug per million cells for 30 minutes, followed by Fluoro488-conjugated secondary antibody. This assay demonstrates intracellular rather than cell-surface staining.

EIF2S3 Antibody by WB in human cell lines. Western blot analysis using Eukaryotic translation initiation factor 2 subunit 3 Antibody detects an immunoreactive band consistent with the expected molecular weight of approximately 51 kDa. Lane assignments: HeLa (lane 1), K562 (lane 2), SiHa (lane 3). Each lane contained 30 ug total protein under reducing conditions, resolved by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.

EIF2S3 Antibody by IHC in human testicular carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human testicular carcinoma using Eukaryotic translation initiation factor 2 subunit 3 Antibody shows brown cytoplasmic staining in a subset of tumor cells. Antigen retrieval was performed with EDTA buffer at pH 8.0. Sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody and DAB chromogen, with hematoxylin counterstaining.