EIF2S1 Antibody (R31138)

EIF2S1 Antibody Human Colon Cancer IHC. IHC staining of FFPE human colon cancer tissue using EIF2S1 Antibody. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. The tissue was incubated with the antibody at 2 ug/ml overnight at 4°C, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Positive EIF2S1 staining is observed in tumor cells. This EIF2S1 Antibody demonstrates eIF2 alpha expression in human colon cancer tissue, supporting studies of translation control and cellular stress signaling.

EIF2S1 Antibody Human, Mouse and Rat WB. Western blot analysis of EIF2S1 in human MCF-7, Jurkat, HepG2 and HeLa cells, rat PC-12 cells, mouse brain tissue and mouse NIH3T3 cells using EIF2S1 Antibody. Samples (30 ug/lane) were separated by 10% SDS-PAGE under reducing conditions and transferred to nitrocellulose. Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: HepG2; Lane 4: HeLa; Lane 5: PC-12; Lane 6: mouse brain; Lane 7: NIH3T3. The membrane was incubated with the antibody at 0.5 ug/ml overnight at 4°C, followed by HRP-conjugated secondary antibody and ECL detection. A specific band was detected at approximately 38 kDa, close to the predicted 36 kDa molecular weight of EIF2S1. This EIF2S1 Antibody detects eIF2 alpha across human, mouse and rat samples for studies of translation control and cellular stress signaling.