EIF2B4 Antibody / Translation initiation factor eIF2B subunit delta Antibody (FY13583)

EIF2B4 Antibody by FACS in K562 cells. Flow cytometric analysis using Translation initiation factor eIF2B subunit delta Antibody shows a rightward shift in the blue antibody-stained population compared with the green rabbit IgG isotype control and red unstained control. K562 cells were fixed with 4% paraformaldehyde and permeabilized for intracellular staining, then blocked with 10% normal goat serum. Cells were incubated with 1 ug antibody per 1 x 10^6 cells for 30 minutes at 20oC, followed by Fluoro488-conjugated secondary antibody. This assay measures intracellular staining in fixed, permeabilized cells rather than live-cell surface expression.

EIF2B4 Antibody by WB in human cell lines and rodent testis. Western blot analysis using Translation initiation factor eIF2B subunit delta Antibody detects a band near 58 kDa in human MCF-7 (lane 1), K562 (lane 2), HeLa (lane 3), rat testis (lane 4), and mouse testis (lane 5). The calculated molecular weight is approximately 58 kDa. Reduced samples containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.

EIF2B4 Antibody by IP in K562 cells. Immunoprecipitation using Translation initiation factor eIF2B subunit delta Antibody was evaluated by Western blot. Lane 1 contains K562 input lysate (30 ug), lane 2 is the rabbit IgG immunoprecipitation control, and lane 3 contains protein precipitated from 500 ug K562 lysate with 2 ug antibody. A band near the expected 58 kDa position is present in the antibody immunoprecipitate. The immunoprecipitated material was analyzed with the same primary antibody at 0.5 ug/mL, followed by an HRP-conjugated secondary antibody and chemiluminescent detection.