- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
EIF2A Antibody / Non-Canonical Translation Initiation and Stress Adaptation Marker (FY13068)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY13068 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 449 |
| Availability | 1-2 days |
| Species Reactivity | Human, Rat |
| Format | Lyophilized |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Immunogen affinity purified |
| Buffer | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| UniProt | Q9BY44 |
| Localization | Cytoplasm |
| Applications | Western Blot : 0.25-0.5ug/ml Immunohistochemistry : 2-5ug/ml Flow Cytometry : 1-3ug/million cells ELISA : 0.1-0.5ug/ml |
| Limitations | This EIF2A Antibody / Non-Canonical Translation Initiation and Stress Adaptation Marker is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Eukaryotic initiation factor 2A (EIF2A) is a specialized translation initiation factor that functions in non-canonical protein synthesis pathways, particularly under conditions where classical cap-dependent translation is impaired. EIF2A Antibody is used to detect this protein, which supports alternative mechanisms of translation initiation and contributes to cellular adaptation during stress. Importantly, EIF2A is distinct from EIF2S1 (eIF2 alpha), a component of the canonical eIF2 complex, and operates through separate molecular mechanisms.
Under normal conditions, protein synthesis is primarily mediated by the eIF2 complex, which delivers initiator methionyl-tRNA to the ribosome in a GTP-dependent manner. During cellular stress, EIF2S1 is phosphorylated, leading to inhibition of this pathway and a reduction in global protein synthesis. EIF2A provides an alternative mechanism that bypasses this inhibition, allowing selective translation of specific mRNAs even when canonical initiation is suppressed. This function is critical for maintaining expression of proteins required for stress response, survival, and adaptation.
EIF2A has been shown to facilitate translation initiation at non-AUG start codons and to support synthesis of proteins involved in regulatory and stress-related pathways. Its activity is particularly relevant in conditions such as endoplasmic reticulum stress, viral infection, and nutrient deprivation, where cells must reprogram protein synthesis to adapt to environmental challenges. Through these mechanisms, EIF2A contributes to fine-tuning of gene expression under conditions where global translation is restricted.
Subcellularly, EIF2A is predominantly localized in the cytoplasm, where it associates with ribosomes and translation machinery. Immunofluorescence studies typically reveal diffuse cytoplasmic staining, sometimes with enrichment in perinuclear regions or areas of active protein synthesis. Its localization may shift under stress conditions, reflecting its involvement in selective translation and adaptive cellular responses.
Unlike EIF2S1, which regulates global translation rates through phosphorylation-dependent inhibition, EIF2A functions as a complementary factor that sustains protein synthesis under restrictive conditions. Detection of EIF2A therefore provides insight into alternative translation pathways and cellular resilience mechanisms. This distinction is important for interpreting experimental results, particularly in studies examining stress signaling and translational control.
EIF2A expression has been observed across a range of tissues and cell types, consistent with its role in maintaining adaptive protein synthesis. Dysregulation of EIF2A-mediated translation has been implicated in cancer and stress-associated signaling pathways, where selective protein production can support tumor survival and progression. These features support the use of an EIF2A Antibody in studies of non-canonical translation initiation, stress adaptation, and regulation of protein synthesis under challenging cellular conditions.
The use of an EIF2A Antibody enables investigation of alternative translation initiation mechanisms that operate independently of the canonical eIF2 complex. Its detection provides valuable insight into translational reprogramming, cellular stress responses, and the balance between global translational repression and selective protein synthesis.
This antibody is part of a broader antibody panel offered by NSJ Bioreagents.
Optimal dilution of the EIF2A Antibody / Non-Canonical Translation Initiation and Stress Adaptation Markery should be determined by the researcher.
E.coli-derived human EIF2A recombinant protein (Position: H24-I585) was used as the immunogen for the EIF2A antibody.
After reconstitution, the EIF2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
EIF2A antibody, Eukaryotic initiation factor 2A antibody, eIF2A protein antibody, Alternative initiation factor EIF2A antibody, EIF2A translation factor antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
