EID3 Antibody / EP300-interacting inhibitor of differentiation 3 Antibody (FY13649)

EID3 Antibody by FCM in K562 cells. Flow cytometry of fixed and permeabilized K562 cells stained with EP300-interacting inhibitor of differentiation 3 Antibody and a Fluoro488-conjugated secondary antibody. The blue antibody-stained population is shifted to higher fluorescence than the green rabbit IgG isotype control, while the red blank control remains at lower fluorescence. Cells were blocked with 10% normal goat serum, stained with 1 ug primary antibody per million cells and analyzed for intracellular immunoreactivity.

EID3 Antibody by IF in A549 cells. Immunofluorescence of A549 cells using EP300-interacting inhibitor of differentiation 3 Antibody shows a green signal concentrated in nuclei, alongside red alpha tubulin staining in the cytoplasm. Cells were treated with enzyme antigen retrieval reagent, blocked with 10% goat serum and incubated with 5 ug/ml primary antibody overnight at 4oC. Fluoro488 anti-rabbit and Cy3 anti-mouse secondary antibodies were used to visualize the two primary antibodies.

EID3 Antibody by WB in human and rodent samples. Western blot analysis using EP300-interacting inhibitor of differentiation 3 Antibody on reducing lysates from HeLa cells (lane 1), A549 cells (lane 2), rat testis (lane 3) and mouse testis (lane 4). Samples were separated on a 10% SDS-PAGE gel and transferred to nitrocellulose. The principal immunoreactive band migrates at approximately 38 kDa, matching the predicted molecular weight of EID3. Primary antibody was applied at 0.5 ug/ml overnight at 4oC, followed by HRP-conjugated goat anti-rabbit IgG and chemiluminescent detection.