EGR3 Antibody / Early growth response protein 3 Antibody (FY13522)

EGR3 Antibody by IHC in human colon carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human colon carcinoma using Early growth response protein 3 Antibody shows brown immunoreactivity in numerous tumor cells, with staining visible in nuclear and perinuclear regions. Hematoxylin counterstaining identifies nuclei and tissue architecture. Sections underwent heat-mediated antigen retrieval in EDTA buffer, pH 8.0, followed by incubation with primary antibody at 2 ug/mL overnight at 4oC. HRP-based detection with DAB produced the brown reaction product. This field demonstrates EGR3 immunoreactivity in a densely cellular tumor region.

EGR3 Antibody by WB across human and rodent samples. Western blot analysis with Early growth response protein 3 Antibody shows a major band near 43 kDa in human Jurkat (lane 1), THP-1 (lane 2), PC-3 (lane 3), and MCF-7 (lane 4) cell lysates, rat testis (lane 5), rat brain (lane 6), mouse testis (lane 7), and mouse brain (lane 8). The observed migration is consistent with the approximately 43 kDa calculated molecular weight of EGR3. Samples contained 30 ug of reduced protein per lane, resolved by 10% SDS-PAGE. Primary antibody incubation was performed at 0.5 ug/mL overnight at 4oC before chemiluminescent detection.

EGR3 Antibody by IHC in human testicular carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human testicular carcinoma using Early growth response protein 3 Antibody reveals variable brown staining in tumor cells, including conspicuous nuclear-associated and cytoplasmic signal in parts of the field. Blue hematoxylin counterstaining distinguishes cell nuclei from the DAB reaction product. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8.0, followed by overnight incubation with primary antibody at 2 ug/mL at 4oC. This section provides an additional tumor-tissue example of EGR3 immunoreactivity.

EGR3 Antibody by IHC in rat brain. Immunohistochemistry of formalin-fixed, paraffin-embedded rat brain using Early growth response protein 3 Antibody shows scattered brown-positive cells against a comparatively lightly stained neural tissue background. Hematoxylin counterstaining highlights nuclei throughout the section. Antigen retrieval was performed in EDTA buffer, pH 8.0, and sections were incubated with primary antibody at 2 ug/mL overnight at 4oC, followed by HRP/DAB detection. The heterogeneous staining pattern illustrates EGR3 immunoreactivity in selected cells within the examined brain region.