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Home >> Antibodies >> EGFR Antibody

EGFR Antibody [clone AEF-5] (RQ4838)

  Catalog No Formulation Size Price (USD)  
Image RQ4838 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of human HeLa cell lysate with EGFR antibody. Expected molecular weight: 134-170 kDa depending on glycosylation level.
IHC staining of FFPE human stomach cancer with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name AEF-5
Purity Affinity purified
UniProt P00533
Localization Cytoplasmic
Applications Western blot : 1:5000-1:10000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This EGFR antibody is available for research use only.
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Description

EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.

Application Notes

Optimal dilution of the EGFR antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human EGFR was used as the immunogen for the EGFR antibody.

Storage

Store the EGFR antibody at -20oC.

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