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Home >> Antibodies >> EGFR Antibody (cytoplasmic domain)

EGFR Antibody (cytoplasmic domain) [clone H9B4] (V2107)

  Catalog No Formulation Size Price (USD)  
Image V2107-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V2107-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V2107SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
Western blot testing of A431 lysate using EGFR antibody at 1ug/ml. Expected molecular weight: 134-180 kDa depending on glycosylation level.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using EGFR antibody (clone H9B4). These results demonstrate the foremost specificity of the H9B4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free EGFR antibody (clone H9B4) as confirmation of integrity and purity.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name H9B4
Purity Protein G affinity chromatography
Gene ID 1956
Localization Cell surface
Applications Immunoprecipitation : 1-2ug/500ug protein lysate
Western blot : 0.5-1ug/ml for 2 hours at RT
Limitations This EGFR antibody is available for research use only.
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Description

Epidermal Growth Factor Receptor is a type I receptor tyrosine kinase, referred to as EGFR, ErbB1 and HER1. When EGFR is activated by one of its ligands, it dimerizes. It can form a homodimer, heterodimers with other ErbB family members, or even a cluster of EGFRs. Activation stimulates EGFRs intracellular kinase activity. Autophosphorylation of tyrosine residues in the C-terminal domain of EGFR leads to association with proteins with phosphotyrosine-binding domains which then signal the initiation of signal transduction cascades including the JNK, MAPK, AKT, and possibly�Nf-KB, pathways. Overexpression of EGFR is the cause of some types of cancer, including lung and colon cancer. It has also been linked to psoriasis, eczema and atherosclerosis, although poorly defined. Monoclonal antibody to EGFR can be used to block the extracellular ligand binding domain, therebye blocking tyrosine kinase activation and subsequent signal transduction.

Application Notes

Titration of the antibody may be required for optimal performance.

1. This antibody reacts with a cytoplasmic domain of EGFR.

Immunogen

Purified EGFR from A431 cells was used as the immunogen for this antibody. (1)

Storage

Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5, ERBB1 antibody

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