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Home >> Antibodies >> EGFR Antibody

EGFR Antibody [clone GFR1195] (V2487)

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  Catalog No Formulation Size Price (USD)  
Image V2487-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2487-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2487SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2487IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC: Formalin-fixed, paraffin-embedded human placenta stained with EGFR antibody (clone GFR1195).
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IHC: Formalin-fixed, paraffin-embedded human placenta stained with EGFR antibody (clone GFR1195).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using EGFR antibody (clone GFR1195). These results demonstrate the foremost specificity of the GFR1195 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free EGFR antibody (clone GFR1195) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name GFR1195
Purity Protein G affinity chromatography
UniProt P00533
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This EGFR antibody is available for research use only.
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Description

This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Application Notes

Optimal dilution of the EGFR antibody should be determined by the researcher.

1. Digest formalin-fixed tissues with Protease at 1mg/ml PBS, pH 7.4 for 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human EGF Receptor protein was used as the immunogen for the EGFR antibody.

Storage

Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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   1.   View all EGFR antibodies

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