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Home >> Antibodies >> EGFR Antibody / EGF Receptor

EGFR Antibody / EGF Receptor [clone GFR450] (V2105CF488)

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  Catalog No Formulation Size Price (USD)  
Image V2105CF488-100T 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 Tests 539
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Left: Immunofluorescent staining of human A431 cells with CF488-conjugated EGFR antibody (clone GFR450, green) and DAPI nuclear stain (blue). Right: isotype control.
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Left: Immunofluorescent staining of human A431 cells with CF488-conjugated EGFR antibody (clone GFR450, green) and DAPI nuclear stain (blue). Right: isotype control.
Flow cytometry testing of human A431 cells with EGFR antibody (clone GFR450); Black=cells alone, Gray=isotype control, Green= EGFR antibody.
Flow cytometry testing of mouse NIH3T3 cells with EGFR antibody (clone GFR450); Black=cells alone, Gray=isotype control, Green= EGFR antibody. This antibody is not suitable for mouse sample testing.
Availability 1-3 business days
Species Reactivity Human (not suitable for mouse sample testing)
Format CF488 Conjugate
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name GFR450
Purity Protein G affinity chromatography
UniProt P00533
Applications Flow Cytometry : 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood
Immunofluorescence : 1:50-1:100
Limitations This EGFR antibody is available for research use only.
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Description

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Application Notes

Optimal dilution of the EGFR antibody should be determined by the researcher.

Immunogen

Recombinant extracellular domain of human EGFR protein was used as the immunogen for the EGFR antibody.

Storage

Store the EGFR antibody at 2-8oC, protected from light.

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