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Home >> Antibodies >> EGF Receptor Antibody

EGF Receptor Antibody [clone B1D8] (V2106)

  Catalog No Formulation Size Price (USD)  
Image V2106-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2106-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2106SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name B1D8
Purity Protein G purified monoclonal antibody
Buffer 1X PBS, pH 7.4
Gene ID 1956
Localization Cell surface
Applications Affinity purification (order BSA/sodium azide-free format for coupling) :
Blocks ligand-induced activation of EGFR :
Inhibits proliferation of A431 cells (order BSA/azide-free format) :
Arrests tumor growth in vivo (order BSA/azide-free format) :
Immunoprecipitation : 1-2ug/500ug protein lysate
Flow cytometry : 0.5-1ug/10^6 cells
Limitations This EGF Receptor antibody is available for research use only.
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Description

This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Immunogen

Microsomes from A431 cells were used as the immunogen for this antibody.

Storage

Store the EGF Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

ErbB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB1; Receptor Tyrosine Protein Kinase; Urogastrone; Wa2; Wa5, EGF Receptor antibody

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