EGFR Antibody [clone AEF-5] (RQ4838)

EGFR Antibody WB. Western blot testing of human HeLa cell lysate with EGFR antibody. Expected molecular weight: 134-170 kDa depending on glycosylation level.

EGFR Antibody Stomach Cancer IHC. Immunohistochemistry staining of FFPE human stomach cancer with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

EGFR Antibody Thyroid Cancer IHC. Immunohistochemistry staining of FFPE human thyroid cancer with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

EGFR Antibody Rat Stomach IHC. Immunohistochemistry staining of FFPE rat stomach tissue with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

EGFR Antibody Mouse Intestine IHC. Immunohistochemistry staining of FFPE mouse intestine tissue with EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

EGFR Antibody HeLa IF. Immunofluorescent staining of FFPE human HeLa cells with EGFR antibody at 1:50 dilution (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

EGFR Antibody FFPE HeLa Cell IF. Immunofluorescent staining of FFPE human HeLa cells with EGFR antibody at 1:150 dilution (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

EGFR Antibody Human HeLa Cell IF. Immunofluorescent staining of FFPE human HeLa cells with EGFR antibody at 1:1500 dilution (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

EGFR Antibody Multi-Sample WB. Western blot analysis of human and rodent lysates using EGFR antibody detecting Epidermal growth factor receptor. Lane 1: HeLa, Lane 2: A431, Lane 3: A549, Lane 4: U-87 MG, Lane 5: rat liver, Lane 6: mouse liver. A prominent band is observed at approximately 140-170 kDa, consistent with the predicted molecular weight of EGFR and its known glycosylation. Signal intensity is highest in A431 cells, reflecting elevated EGFR expression. Additional lower molecular weight bands and background signal are present, which may represent degradation products or non-specific binding.