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Home >> Antibodies >> EGFR Antibody

EGFR Antibody [clone 51CT78.40.5] (F40415)

  Catalog No Formulation Size Price (USD)  
Image F40415-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 379
Image
F40415-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot analysis of lysate from A431, MDA-MB-468 cell line (left to right) using EGFR antibody at 1:1000 for each lane. Expected molecular weight: ~134/170 kDa (unmodified/glycosylated).
IHC analysis of FFPE human breast carcinoma with EGFR antibody
Flow cytometric analysis of HepG2 cells using EGFR antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, k
Clone Name 51CT78.40.5
Purity Purified
UniProt P00533
Localization Cytoplasmic, membranous
Applications Western blot : 1:2000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This EGFR antibody is available for research use only.
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Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Application Notes

Titration of the EGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Purified His-tagged protein fragment was used to produced this monoclonal EGFR antibody.

Storage

Aliquot the EGFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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