EGFL7 Antibody / EGF-like domain-containing protein 7 [clone EGFL7/9497] (V5965)

Immunohistochemistry analysis of EGFL7 / EGF-like domain-containing protein 7 antibody in human tonsil. FFPE human tonsil tissue was stained with EGFL7 / EGF-like domain-containing protein 7 antibody (clone EGFL7/9497). HRP-DAB brown chromogenic signal is observed predominantly along the endothelial lining of vascular structures and within the extracellular matrix surrounding vessels, consistent with the known extracellular and endothelial-associated localization of EGFL7. Vascular endothelial cells display membranous and cytoplasmic brown staining, while adjacent lymphoid cells show minimal background signal. Nuclei are counterstained blue. The inset image shows PBS used instead of primary antibody, demonstrating absence of specific HRP-DAB brown staining and confirming staining specificity. Heat-induced epitope retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to antibody incubation.

Western blot analysis of EGFL7 / EGF-like domain-containing protein 7 antibody in human tissues and cell lines. Western blot was performed using EGFL7 / EGF-like domain-containing protein 7 antibody (clone EGFL7/9497) on human kidney, human lung, human spleen, HL60, K-562, and MCF7 lysates. A distinct immunoreactive band is detected at approximately 27 kDa across the tested samples, consistent with the predicted molecular weight of EGFL7. Band intensity varies among tissues and cell lines, reflecting differential expression of EGFL7 in vascularized tissues and proliferative cell populations. The detected signal corresponds to the predicted molecular weight under reducing conditions. EGFL7 is a secreted extracellular matrix-associated protein primarily expressed by endothelial cells, and its detection in these lysates supports specific recognition of endogenous EGFL7.

Western blot analysis of EGFL7 / EGF-like domain-containing protein 7 antibody in human 293T cell lysate. A distinct immunoreactive band is detected at approximately 27 kDa, consistent with the predicted molecular weight of EGFL7.

SDS-PAGE Analysis Purified EGFL7/EGF-like domain-containing protein 7 antibody (EGFL7/9497). Confirmation of Purity and Integrity of Antibody.